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TEMIMPS Staff:
At TEMIMPS significant emphasis is devoted to attracting biologists with scientific questions that can be addressed by electron crystallography of membrane proteins. TEMIMPS will carry out 2D crystallization and structure determination of membrane protein targets from collaborators.



Andreas Schenk, Ph.D.
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Postdoctoral Associate
Harvard Medical School




Stephanie Hsiao, Ph.D.
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Postdoctoral Associate
Harvard Medical School




Maria Lopez Redondo, Ph.D.
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Postdoctoral Associate
New York University School of Medicine
Marisa received her Ph.D. from the Institute of Biomedicine in Valencia Spain, where she used X-ray crystallography and computational biology to study the soluble domains of two-component systems. She joined the Stokes lab in 2011 and is using methods of electron crystallography in order to study the structure of membrane-bound two-component systems and the complexes they form with their response regulators and adaptor proteins.




Ardeschir Vahedi-Faridi, Ph.D.
Postdoc
Case Western Reserve University
Ardeschir Vahedi-Faridi joined Andreas Engel at the Case Western Reserve University in May 2011. Ardi received his MS in Chemistry at the Ludwig Maximilians University, Munich, Germany, and completed his Ph.D. thesis 'X-ray Structure of Clay-Organic Intercalates' at the University of Illinois, Chicago in 1998. He moved then into biology, working on improving crystallization conditions in the Department of Chemistry, University of Toledo. In pursuit of his interest in protein structure he spent five years in Wolfram Sänger's Laboratory at the Free University Berlin. Ardi is interested in electron crystallography of membrane proteins and in technical developments.



George Wisedchaisri, Ph.D.
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Postdoc
Janelia Farms, HHMI Medical Institute
George Wisedchaisri joined Tamir Gonen's Laboratory of Molecular Electron Microscopy at the University of Washington in 2006 and is now working in Tamir's laboratory at the Janelia Farms campus of HHMI Medical Institute. George received his Ph.D. from the University of Washington where his projects included structural studies of several protein-DNA complexes by X-ray crystallography and drug designs against a tropical disease. In the Gonen laboratory, George is currently developing new methodologies for electron diffraction data processing. His recent work includes the development of the fragment-based phase extension method for structure determination of membrane proteins by electron crystallography. He is collaborating with the Baker laboratory to completely automate the procedure.



Jose Chavez, Ph.D.
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Postdoc
Mount Sinai School of Medicine
Jose joined the TEMIMPS consortium in November 2010. During his PhD at the City University of New York (New York, New York), his research was focused on enzymatic characterization of rhomboid intra-membrane proteases. In the frame of his research he developed methods for the evaluation of enzymatic activity and the determination of the cleavage site using techniques such as affinity chromatography and MALDI-TOF mass spectrometry for rhomboid. At Mount Sinai School of Medicine he will be in charge of the expression and purification of membrane proteins to be used in 2D-crystallization and biochemical characterization.



Nanda Lahiri
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Technician
Mount Sinai School of Medicine
Nanda joined the TEMIMPS consortium in December 2010. She has previously worked on studying the effects of an experimental drug on various cancer cell lines, focusing on proteins involved in cell stress and apoptosis. At Mount Sinai School of Medicine she will be involved in the expression and purification of membrane proteins to be used in 2D-crystallization and biochemical characterization.



Nathan Will
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Technician
Mount Sinai School of Medicine
Nathan joined the TEMIMPS consortium in November 2010. Previous work includes purification of Brain Derived Neurotrophic Factor and analyzing its interaction with Sortilin Family members. At Mount Sinai School of Medicine he will focus on the expression and purification of membrane proteins to be used in 2D-crystallization and biochemical characterization.



Nicolas Coudray, Ph.D.
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Project Manager
New York Structural Biology Center
Nicolas joined the TEMIMPS consortium in November 2010. During his PhD at the Université de Haute Alsace (Mulhouse, France), his research focused on the automation of a TEM for the evaluation of 2D-crystallization trials. He developed image processing tools to characterize samples and to select targets for electron microscope control (as part of the HT-3DEM project). At NYSBC, he will be involved in crystallization trials, sample screening, and software development, among other things.




Ralph Lasala, Ph.D.
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Postdoc
New York Structural Biology Center
Ralph joined TEMIMPS in December 2010. During his PhD studies with Lynne Cox (University of Oxford) and Robert Saunders (The Open University), he carried out genetic and biochemical experiments to examine the biological role of Drosophila WRN exonuclease (DmWRNexo). He also did structural characterisation of DmWRNexo by single particle electron microscopy. At NYSBC, he is involved in 2D electron crystallography and database contribution to PSI's SBKB.



Salvatore Valvo
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Technician
NYU Medical School
Salvatore joined the TEMIMPS consortium in November 2010. At NYU he was previously involved in work including Desmoplakin mediated anchoring of intermediate filaments to desmosomes. He studued the molecular organization of desmosomes by electron tomography and characterized the structural consequences of desmoplakin knockout and wild-type desmosomes. Currently he is involved on the expression and purification of membrane proteins, as well as their 2D-crystallization and biochemical characterization.



Tao Juoehi, Ph.D.
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Senior Ph.D. Associate
Univeristy of Texas
Houston Medical Center
Guozhi Tao is now working as a research scientist at Department of Biochemistry and Molecular Biology at University of Texas-Houston Medical School. He received Ph.D degree in electrical engineering from University of Houston, Houston TX in 2006. His research interests include three dimensional medical image segmentation, nonrigid registration as well their applications on CT and MR images. His current research interest focuses on single particle reconstruction from Electron Microscopy.









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